The induction of nitrite reductase in Neurospora crassa.
نویسنده
چکیده
Results with strain am-la, a glutamate dehydrogenaseless mutant, showed that ammonium ions must first be metabolized in order to repress nitrite reductase in Neurospora.
منابع مشابه
Siroheme: a prosthetic group of the Neurospora crassa assimilatory nitrite reductase.
The Neurospora crassa assimilatory nitrite reductase (EC 1.6.6.4) catalyzes the NADPH-dependent reduction of nitrite to ammonia, a 6-electron transfer reaction. Highly purified preparations of this enzyme exhibit absorption spectra which suggest the presence of a heme component (wavelength maxima for oxidized senzyme: 390 and 578 nm). There is a close correspondence between nitrite reductase ac...
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Like the nitrate transport system, the nitrite uptake system in Neurospora crassa is induced by either nitrate or nitrite. This induction is prevented by cycloheximide, puromycin, or 6-methyl purine. The K(m) for nitrite of the induced nitrite uptake system is 86 muM, and the V(max) is 100 mumol of nitrite per g (wet weight) per h. Nitrite uptake is inhibited by metabolic poisons such as arsena...
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The enzyme systems which catalyze the reduction of nitrate to nitrite have generally been classified into two broad types: (a) assimilatory nitrate reductase, which is responsible for the first step in the reduction of nitrate for the ultimate biosynthesis of nitrogen-containing cell constituents (e.g., amino acids aind purines and pyrimidines), and (b) respiratory or dissimilatory nitrate redu...
متن کاملNitrate transport system in Neurospora crassa.
Nitrate uptake in Neurospora crassa has been investigated under various conditions of nitrogen nutrition by measuring the rate of disappearance of nitrate from the medium and by determining mycelial nitrate accumulation. The nitrate transport system is induced by either nitrate or nitrite, but is not present in mycelia grown on ammonia or Casamino Acids. The appearance of nitrate uptake activit...
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Enzymes capable of catalyzing the hydroxylamine-dependent. oxidation of pyridine nucleotides have been studied in Salmonella typhimurium (1, 2), Escherichia coli (3-5), Bacillus subtilis (6, 7), Azotobacter agile (8, 9), Neurospora crassa (lo), and soybean leaves (11). Siegel, Click, and Monty (1) and Siegel and Monty (2) demonstrated that reduced triphosphopyridine nucleotide-specific hydroxyl...
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ورودعنوان ژورنال:
- Biochimica et biophysica acta
دوره 264 3 شماره
صفحات -
تاریخ انتشار 1972